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laser microdissection platform  (Inserm Transfert)

 
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    Inserm Transfert laser microdissection platform
    Laser Microdissection Platform, supplied by Inserm Transfert, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/laser+microdissection+platform/pm38986099-119-2-15?v=Inserm+Transfert
    Average 90 stars, based on 1 article reviews
    laser microdissection platform - by Bioz Stars, 2026-07
    90/100 stars

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    Image Search Results


    Novel laser model allows for accurate and reproducible optic nerve transections. A) Leica LMD7 laser microdissection experimental setup with Xla.Tg(tubb2b: mapt-GFP) Amaya transgenic X. laevis line. A’) Tadpoles are anesthetized and loaded onto small surgical beds made of 4% agarose inside a chamber slide, so they are flat with their dorsal side up. B) Pre-operative and post-operative views of the endogenously labelled optic nerve in the GFP channel. The gap in fluorescence in the post-operative image indicates the nerve was properly transected. C) Representative images of the precision and reproducibility of this model in 3 tadpoles with their nerves cut at the same distance from the eye. D) Longitudinal imaging of a tadpole after laser optic nerve transection. Axons distal to the injury site degenerate from day 0–2 post laser (PL), followed be several axons sprouting from the proximal cut end in all directions from the injury site by day 3 PL. Some regrowing axons quickly reach the brain after which this connection becomes reinforced by additional axons that follow the path of those pioneer axons. After 14 days, the optic nerve is virtually indistinguishable from the naïve (uninjured) optic nerve morphology.

    Journal: Scientific Reports

    Article Title: Novel laser model of optic nerve transection provides valuable insights about the dynamics of optic nerve regeneration

    doi: 10.1038/s41598-024-79296-0

    Figure Lengend Snippet: Novel laser model allows for accurate and reproducible optic nerve transections. A) Leica LMD7 laser microdissection experimental setup with Xla.Tg(tubb2b: mapt-GFP) Amaya transgenic X. laevis line. A’) Tadpoles are anesthetized and loaded onto small surgical beds made of 4% agarose inside a chamber slide, so they are flat with their dorsal side up. B) Pre-operative and post-operative views of the endogenously labelled optic nerve in the GFP channel. The gap in fluorescence in the post-operative image indicates the nerve was properly transected. C) Representative images of the precision and reproducibility of this model in 3 tadpoles with their nerves cut at the same distance from the eye. D) Longitudinal imaging of a tadpole after laser optic nerve transection. Axons distal to the injury site degenerate from day 0–2 post laser (PL), followed be several axons sprouting from the proximal cut end in all directions from the injury site by day 3 PL. Some regrowing axons quickly reach the brain after which this connection becomes reinforced by additional axons that follow the path of those pioneer axons. After 14 days, the optic nerve is virtually indistinguishable from the naïve (uninjured) optic nerve morphology.

    Article Snippet: To develop a new surgical model, we used the Leica LMD7 laser microdissection platform and Xla.Tg (tubb2b: mapt-GFP) Amaya transgenic X. laevis line to visualize the optic nerve cut and the subsequent axonal regrowth.

    Techniques: Laser Capture Microdissection, Transgenic Assay, Fluorescence, Imaging